Volume 2, Issue 5, September 2016, Page: 75-78
Isolation and Identification of Seed Borne Fungi of Common Bean (Phaseolus vulgaris L.) from Selected Markets in Makurdi
Liamngee Kator, Department of Biological Sciences, Benue State University, Makurdi, Nigeria
Aondo Terkaa Ogo-Oluwa, Department of Biological Sciences, Benue State University, Makurdi, Nigeria
Akinyemi Bosede Kemi, Department of Biological Sciences, Benue State University, Makurdi, Nigeria
Received: Jun. 6, 2016;       Accepted: Jun. 29, 2016;       Published: Aug. 4, 2016
DOI: 10.11648/j.ijaas.20160205.11      View  4829      Downloads  249
Abstract
Isolation and identification of seed borne fungi of common bean (Phaseolus vulgaris) from selected markets in Makurdi was conducted. A total of four fungi namely Aspergillus niger, Aspergillus flavus, Fusarium oxysporum and Botryodiplodia theobromae were isolated from the bean samples. Percentage germination /viability of the samples ranged from 33-53%. There was no significant difference at P=0.05 in the viability of seeds from North bank and Wadata markets while seeds from Wurukum and High level markets showed significant difference (P < 0.05) in viability. Percentage of seeds infected with fungi ranged from 33-67% in all sample locations. There was no significant difference (P > 0.05) in the percentage number of seeds infected with fungi. The percentage of fungi isolated from samples ranged from 50-100% in all sampled sites. Analysis of Variance revealed no significant difference (P > 0.05) in the percentage of fungi on the bean samples. Frequency of occurrence of each fungi isolated ranged from 8.35-47.7%. There was no significant difference (P > 0.05) in the frequency of occurrence of fungi isolated from the samples. Isolation of fungal pathogens from the bean seeds indicate that they should be treated before sowing to obtain good germination and healthy crop.
Keywords
Isolation, Identification, Seed Borne Fungi, Common Bean, Makurdi
To cite this article
Liamngee Kator, Aondo Terkaa Ogo-Oluwa, Akinyemi Bosede Kemi, Isolation and Identification of Seed Borne Fungi of Common Bean (Phaseolus vulgaris L.) from Selected Markets in Makurdi, International Journal of Applied Agricultural Sciences. Vol. 2, No. 5, 2016, pp. 75-78. doi: 10.11648/j.ijaas.20160205.11
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
W. J. Broughton, G. Hernandez, M. Beebe, S. Gepts and P. Vanderleyden, “Beans (Phaseolus spp); model food legume,’’ Plant and soil, 252. 55-128, 2003.
[2]
H. F. Schwartz and M. A. Pastor-Carrales. “Bean production problems in the tropics,’’ 2nd Ed. CIAT Cali, Colombia, 1989, Chapter 2, pp 261-282.
[3]
M. Boateng, D. B. Okai, J. Baah and A. Donkoh, “Legume Forage utilization in pig and poultry diets in Ghana,” Livest. Res. Rural Dev. 20 (7): 99, 2008
[4]
National Academy of Sciences, “Genetic vulnerability of Major crops,” Washington, D. C. Tropical Legumes: resources for the future. Washington DC. 1979
[5]
Y. L. Nine, “Opportunities for research on disease of pulse crops,” Indian Phytopathology, 39 (3): 333-335. 1986.
[6]
K. Kubiak and M. Korbas. “Occurrence of fungal diseases in selected winter wheat cultivars,” Postapy Ochronie Roshin, 39 (2): 801, 1999.
[7]
K. H. Domsch, W. Gams and T. H. Anderson, “Compendium of soil fungi,” London, England: Academic press. pp 865, 1980.
[8]
C. Booth, “the genus fusarium,” Survey. UK: CMI, Kew, pp 237, 1971.
[9]
I. S. Ahmed, I. S. Iftikhar and A. R. Bhutta, “Seed Borne Microorganism in Pakistan,” Pakistan agricultural research Council, Islamabad, Pakistan, pp 32.
[10]
K. A. khanzada, M. A. Rajput, G. S. Shah, A. M Lodhi and F. Mehboob. “Effect of seed dressing fungicides for the control of seed borne microflora of wheat,’’ Asian Journal of Plant Sciences, 1 (4): 441-442, 2002.
[11]
A. S Shakir, J. H. Mirza, S. T. Sahi and F. Ahmed, “Detection of seed-borne fungi associated with sponge gourd (Luffa cylindrical (L.) Roem), their location in different seed components and their control,’’ Pak. J. Phytopathol, 7: 140-144, 1995.
[12]
P. Neergard, “Seed pathology,” Vol. 1 and 2, MacMillan Press, London U. K, pp 141-142.
Browse journals by subject